Biopolym. Cell. 2026; 42(2):110-120.
https://doi.org/10.7124/bc.000B3B
Molecular and Cell Biotechnologies
Effect of biogenic AgNPs on the viability and adhesive properties of bacteria
1, 2Fedko M. M., 1Huseinova K. E., 1Potupa V. Yu., 3Shkotova L. V., 1Voloshyna I. M.
  1. Kyiv National University of Technologies and Design
    2, Mala Shyianovska Str., Kyiv, Ukraine, 01011
  2. PHARMHIM, LTD
    1, Industrialna Str., Shostka, Sumy region, Ukraine, 41100
  3. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143

Abstract

Aim. The study demonstrates the efficacy of silver nanoparticles (AgNPs), produced via green synthesis using L. acidophilus UKM B-2691, with regard to the metabolic activity and adhesive properties of Gram-positive and Gram-negative bacteria. Methods. Gram-positive strains (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633) and Gram-negative strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa PA01) were used as test cultures to evaluate the biological activity of biogenic AgNPs within a nanoparticle concentration range of 0.01—10 mM. Results. A correlation was observed between the reduction in metabolic activity and the suppression of adhesion. Particular attention is given to the discovered effect of significant adhesion inhibition at sublethal nanoparticle concentrations, where bacteria maintain high level of metabolic activity but lose the ability to colonize surfaces. It was found that Grampositive test cultures exhibit higher sensitivity to AgNPs within a concentration range of 0.01—10 mM compared to Gram-negative strains. The results indicate the ability of biogenic AgNPs to inhibit early stages of bacterial metabolic activity and significantly reduce their adhesive properties, leading to the suppression of mature biofilm formation. Conclusions. These findings offer promising perspectives for the development of next-generation antimicrobial agents with high biocompatibility.
Keywords: nanosilver, adhesion, antibacterial properties, bacteria
Full text: (PDF, in English)

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