Background/Aim. The rapid spread of antimicrobial resistance necessitates the discovery of novel bioactive compounds for next-generation therapeutics. Marine actinobacteria represent a promising source of structurally diverse secondary metabolites with antimicrobial, antifungal, and anticancer activities. This study aimed to evaluate the antimicrobial activity of extracellular metabolites from marine-derived isolates Streptomyces chumphonensis conc10 against a standardised test cultures and to characterise their secondary metabolome by LC-QTOF-MS. Methods. S. chumphonensis conc10 were isolated from concrete substrates (0.2—1.0 m depth, Odesa Bay, Black Sea; 46°27′01″N, 30°46′14″E; June–July 2020) and cultured on SG medium at 28 °C, 180 rpm for 9 days. Secondary metabolites of S. chumphonensis conc10 were extracted with ethyl acetate and analyzed by LC-QTOF-MS. Putative compound annotation was performed based on accurate mass measurements and metabolite databases. Antimicrobial activity was assessed against Escherichia coli, Staphylococcus aureus, Salmonella enterica, Listeria ivanovii and Lactobacillus sakei. The inhibitory effect was determined using optical density measurements to calculate the bacterial growth inhibition compared to control samples. Results. Antimicrobial screening revealed a selective activity profile of extracellular metabolites produced by S. chumphonensis conc10. The filtered culture supernatant demonstrated the highest inhibitory effect against S. enterica (22.99%) and S. aureus (16.18%), while only weak inhibition was observed against E. coli (4.54%). No inhibitory activity was detected against L. ivanovii. In contrast, the unfiltered supernatant exhibited notable inhibition of S. enterica (18.37%) and E. coli (16.21%), suggesting that both secreted metabolites and cellassociated components may contribute to antimicrobial activity. Metabolomic analysis of S. chumphonensis conc10 revealed putatively identified secondary metabolites, among them, Bacilsubteramide A and Piericidin A5 are known for antimicrobial and cytotoxic activities. The metabolome included 12 compounds previously associated with anticancer properties and 7 metabolites with reported antifungal activity, including Chrysotriazole B. Conclusions. S. chumphonensis conc10 isolated from the Black Sea, demonstrated measurable antimicrobial activity against both gram-positive (S. aureus) and gram-negative (S. enterica, E. coli) bacteria, with the strongest inhibition observed for S. enterica (22.99%). The identification of Piericidin A5 and Chrysotriazole B is of particular translational relevance. The detected metabolite spectrum indicates a high biosynthetic capacity of the strain and suggests the presence of multiple bioactive compound classes with therapeutic potential.