The extreme conditions of the war in Ukraine and breaches of aseptic practice have increased the risk of wound infections with antibioticresistant strains. A comprehensive treatment approach (surgical debridement, combined antibiotic therapy, phage therapy, antiseptics, and reconstructive wound closure) is currently used. A promising strategy is the local application of enzymes and polysaccharides that can disrupt bacterial biofilms and the cell walls of pathogens. Exopolysaccharides (EPS) from lactic acid bacteria are attractive for medical use because they may accelerate tissue regeneration, modulate immune responses, and inhibit pathogenic microorganisms. The aim of the work was to study the antimicrobial activity of EPS produced by two lactobacilli strains from the Collection of Marine and Biotechnology- Useful Microorganisms (Odesa I.I. Mechnikov National University) against both clinical and reference strains of opportunistic bacteria. Methods. Quantitative determination of EPS was performed after the cultivation of Lactobacillus casei subsp. casei L187 OL18 and L. casei subsp. tolerans L290 OL29 in MRS broth with 5% D-sucrose at 24 °C. EPS in culture supernatants were quantified by the phenol-sulfuric acid assay. Antimicrobial activity of aqueous EPS solutions against reference and clinical strains of Pseudomonas aeruginosa and Klebsiella pneumoniae was assessed by the agar-well diffusion method. Antimicrobial susceptibility testing of the clinical isolates (5 strains of P. aeruginosa and 3 strains of K. pneumoniae) was performed using the automated MicroScan WalkAway system (USA). Experiments were performed in three independent replicates. Results. The L. casei strains L187 OL18 and L290 OL29 synthesized 107.5 ± 2.3 mg/mL and 95.6 ± 2.1 mg/mL of EPS, respectively. Clinical strains of P. aeruginosa and K. pneumoniae isolated from combat gunshot wounds were pan-resistant or had extremely low susceptibility to traditional antimicrobial drugs, including beta-lactamase inhibitors, which creates a critically dangerous situation. Clinical isolates of P. aeruginosa and K. pneumoniae from combatrelated gunshot wounds were panresistant or exhibited extremely low susceptibility to conventional antibiotics, including β-lactamase inhibitors. Aqueous solutions of EPS produced by both L. casei strains at a concentration of 0.06 g/L inhibited the growth of collection and clinical strains of P. aeruginosa and K. pneumoniae. The zones of growth inhibition of the collection strains P. aeruginosa ATCC 27853 and K. pneumoniae ATCC 10031 were 10.7 ± 0.3—13.0 ± 0.4 mm when exposed to EPS from both lactobacilli strains. The 8 clinical strains studied were more resistant, with growth inhibition zones of 8.0 ± 0.6—9.2 ± 0.5 mm. The EPS synthesized by the L. casei L290 strain demonstrates consistently higher antimicrobial activity than the EPS of L. casei L187 against all tested bacterial groups. Conclusions. The bacteriostatic effect of EPS produced by the L. casei strains L187 and L290 makes them promising for the creation of local antimicrobial agents. It is advisable to proceed studying the mechanisms of EPS action and their application as a component of combination drugs for the treatment of infected wounds.