Biopolym. Cell. 1989; 5(3):49-53.
Structure and Function of Biopolymers
Affinity modification of chromatin with an alkylating hexadecadeoxyribothymidylate derivative
1Vlassov V. V., 1Kobetz N. D., 1Chernolovskaya E. L., 1Ivanova E. M., 1Subbotin V. M., 1Yakubov L. A.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR


Alkylating derivative of hexadecadeoxyribothymidylate bearing 4-(N-2-chloroethyl-N-methylamino) benzylamine residue on its 5'-terminal phosphate was used for affinity modification of complementary poly (A) sequences in chromatin DNA. The DNA reactivity was not influenced by transcription and replication processes. DNA in suspension chromatin fraction and in intact nuclei was more readily alkylated as compared to that from the soluble chromatin fraction. This effect was attributed to higher superhelicity of DNA in intact nuclear DNA-protein structures. Protein-free isolated DNA was by several orders of magnitude less reactive than the chromatin DNA. Reaction of alkylating oligonucleotide derivatives with DNA is the sequence specific process, since it is suppressed by excess of an arbitrary hexadecanucleotide.


[1] Vlasov VV, Ivanova EM, Kobets ND, Mamaev SV, Shul'zhenko SG. Specific chemical reaction of 5'-alkylating oligodesoxythymidylate derivative with DNA of human placental chromatin. Dokl Akad Nauk SSSR. 1985;282(1):196-9.
[2] Beliaev ND, Vlasov VV, Kobets ND, Ivanova EM, Iakubov LA. Complementary DNA modification in metaphase chromosomes and interphase chromatin. Dokl Akad Nauk SSSR. 1986;291(1):234-6.
[3] Collins JM. Deoxyribonucleic acid structure in human diploid fibroblasts stimulated to proliferate. J Biol Chem. 1977;252(1):141-7.
[4] Carnevali F, Filetici. Chromatine structure. Chromosoma. 1981; 82(2):377-383.
[5] Zarytova VF, Ivanova EM, Romanenko VP. Synthesis of oligonucleotides by phosphotriester approach in chloroform. Russian Journal of Bioorganic Chemistry. 1983; 9 (4):516-21.
[6] Berkner KL, Folk WR. Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA. J Biol Chem. 1977;252(10):3176-84.
[7] Mishenina GF, Samukov VV, Shubina TN. Selective modification of monosubstituted phosphate groups in nucleoside and oligonucleotide 5'-mono- and polyphosphates. Russian Journal of Bioorganic Chemistry. 1979; 5 (6):886-94.
[8] Shick VV, Belyavsky AV, Bavykin SG, Mirzabekov AD. Primary organization of the nucleosome core particles. Sequential arrangement of histones along DNA. J Mol Biol. 1980;139(3):491-517.
[9] William F, Marzluff, Ru Chin C. Huang. Transcription of RNA in isolated nuclei. Transcription and Translation. Ed. B. Hames. Oxford, 1984:89.
[10] Dashkevich VS. Investigation of DNA in cancer cells in the embryonic tissue Usp Sib Otd Akad Nauk SSSR. 1963; 6(1):136-140.
[11] Tereshchenko OD, Khaydarova NV. Eukaryotic DNA topoisomerase: properties and possible functions. Usp Sovrem Biol. 1983; 96(1):28-45.
[12] Levy BW, Connor W, Dixon GH. A subset of trout testis nucleosomes enriched in transcribed DNA sequences contains high mobility group proteins as major structural components. J Biol Chem. 1979;254(3):609-20.
[13] Georgiyev GP, Il'in YuV, Tikhonenko AS, Dobbert NN, Anan'yeva LN. Preparation and properties of chromosomal desoxyribonucleoproteide complexes. Mol Biol (Mosk). 1967; 1(6):815-29.
[14] Vlassov VV, Godovikov AA, Kobetz ND, Ryte AS, Yurchenko LV, Bukrinskaya AG. Nucleotide and oligonucleotide derivatives as enzyme and nucleic acid targeted irreversible inhibitors. Biochemical aspects. Adv Enzyme Regul. 1985;24:301-20.