Biopolym. Cell. 1989; 5(3):54-60.
Structure and Function of Biopolymers
rRNA-protein interactions in Escherichia coli 70S ribosomes and in complex of initiation by data of cross-links with bifunctional Pt(II) derivative
1Chistyakov P. G., 1Abdukayumov M. A., 1Venijaminova A. G., 1Vladimirov S. N., 1Graifer D. M., 1Kazakov S. A., 1Karpova G. G.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR


rRNA-protein cross-links in 70S 35S-labelled ribosomes in the free state and within initiation complex ribosome-AUGU6-fMet-tRNAfMet were investigated using bifunctional cleavable reagent {dichloro [N,N,N',N'-tetrakis- (2-aminoethyl) -1.6-hexamethylendiamine-diplatinum(II)]}dichloride. The sets of proteins cross-linked to 16S and 23S rRNA in free 70S ribosomes and in 70S initiation complex were shown to slightly differ. It was found that one of L7/L12 dimers borders on one of 16S rRNA sites (a distance <5 nm) both in free ribosomes and in initiation complex.


[1] Brimacombe R, Stiege W. Structure and function of ribosomal RNA. Biochem J. 1985;229(1):1-17.
[2] Banjar ZM, Hnilica LS, Briggs RC, Stein J, Stein G. Crosslinking of chromosomal proteins to DNA in HeLa cells by UV gamma radiation and some antitumor drugs. Biochem Biophys Res Commun. 1983;114(2):767-73.
[3] Ciccarelli RB, Solomon MJ, Varshavsky A, Lippard SJ. In vivo effects of cis- and trans-diamminedichloroplatinum(II) on SV40 chromosomes: differential repair, DNA-protein cross-linking, and inhibition of replication. Biochemistry. 1985;24(26):7533-40.
[4] Tukalo MA, Kubler MD, Kern D, Mougel M, Ehresmann C, Ebel JP, Ehresmann B, Giege R. trans-Diamminedichloroplatinum(II), a reversible RNA-protein cross-linking agent. Application to the ribosome and to an aminoacyl-tRNA synthetase/tRNA complex. Biochemistry. 1987;26(16):5200-8.
[5] Tukalo MA, Rozhko OT, Matsuka GKh, Vlasov VV, Kazakov SA. Platinum derivatives--cross-linking reagents for research on tRNA interaction with aminoacyl-tRNA-synthetases. Dokl Akad Nauk SSSR. 1985;280(6):1484-7.
[6] Ehresmann C, Moine H, Mougel M, Dondon J, Grunberg-Manago M, Ebel JP, Ehresmann B. Cross-linking of initiation factor IF3 to Escherichia coli 30S ribosomal subunit by trans-diamminedichloroplatinum(II): characterization of two cross-linking sites in 16S rRNA; a possible way of functioning for IF3. Nucleic Acids Res. 1986;14(12):4803-21.
[7] Wikman FP, Romby P, Metz MH, Reinbolt J, Clark BF, Ebel JP, Ehresmann C, Ehresmann B. Crosslinking of elongation factor Tu to tRNA(Phe) by trans-diamminedichloroplatinum (II). Characterization of two crosslinking sites in the tRNA. Nucleic Acids Res. 1987;15(14):5787-801.
[8] Vladimirov SN, Graifer DM, Zenkova MA, Kazakov SA, Karpova GG. The use of cross-linking platinum reagents in the study of tRNA and mRNA interaction with ribosomes. Bioorg Khim. 1987;13(8):1053-8.
[9] Abdurashidova GG, Nargizian MG, Rudenko NV, Turchinskii MF, Budovskii EI. Contacts of ribosomal proteins with tRNAPhe and 16S RNA in analogs of the 30S initiation complex. Mol Biol (Mosk). 1985;19(2):553-7.
[10] Abdurashidova GG, Tsvetkova EA, Chernyi AA, Kaminir LB, Budowsky EI. Intersubunit RNA-protein contacts in pre- and post-translocated E. coli ribosome. FEBS Lett. 1985;185(2):291-4.
[11] Abdukaiumov M, Gimautdinova OI, Graifer DM, Zenkova MA, Karpova GG. Preparation of Escherichia coli 70S ribosomes labeled with 35S. Biokhimiia. 1987;52(9):1411-6.
[12] Babkina GT, Karpova GG, Berzin' VA, Gren EIa, Tsielens IE. Study of the ribosome mRNA-binding region during different stages of translation. I. Functional activity of mRNA analogs, AUGU6 and its benzylidene derivatives, in ribosome-dependent protein synthesis. Bioorg Khim. 1983;9(11):1535-43.
[13] Vlassov VV, Kazakov SA, Misovets VG. Synthesis of binuclear compounds of Pt (II) with NNN?N?-tetrakis (2-aminoethyl) -1,6-hexamethylenediamine. Dokl Akad Nauk SSSR. 1982; 266(4):866-870.
[14] Gimautdinova OI, Karpova GG, Knorre DG, Kobetz ND. The proteins of the messenger RNA binding site of Escherichia coli ribosomes. Nucleic Acids Res. 1981;9(14):3465-81.
[15] RNA structure and RNA-protein neighbourhoods in the ribosome. R. Brimacombe, J. Atmadja, A. Kvriatsotilis. Wh Stieee. Structure, function and genetics of ribosomes . Eds B. Hardcsty, G. Kramer - New York: Springer, 1986:184-202.
[16] Budowsky EI, Axentyeva MS, Abdurashidova GG, Simukova NA, Rubin LB. Induction of polynucleotide-protein cross-linkages by ultraviolet irradiation. Peculiarities of the high-intensity laser pulse irradiation. Eur J Biochem. 1986;159(1):95-101.
[17] Golinska B, Millon R, Backendorf C, Olomucki M, Ebel JP, Ehresmann B. Identification of a 16-S RNA fragment crosslinked to protein S1 within Escherichia coli ribosomal 30-S subunits by the use of a crosslinking reagent: ethyl 4-azidobenzoylaminoacetimidate. Eur J Biochem. 1981;115(3):479-84.
[18] Nitta N, Kuge O, Yui S, Negishi K, Hayatsu H. Cross-linking between 16S ribosomal RNA and protein S4 in Escherichia coli ribosomal 30S subunits effected by treatment with bisulfite/hydrazine and bromopyruvate. Eur J Biochem. 1986;157(2):427-32.
[19] Stofjler G., Stofjler-Meilicke M. Immuno electron microscopy on Escherichia coli ribosomes. Structure, function, and genetics of ribosomes . Eds B. Hardesty, G. Kramer. New York: Springer, 1986:28-46.
[20] Oakes M, Henderson E, Scheinman A, Clark M, Lake JA. Ribosome structure, function, and evolution: mapping ribosomal RNA, proteins, and functional sites in three dimensions. Eds B. Hardesty, G. Kramer. New York: Springer, 1986;47-67.