Biopolym. Cell. 1990; 6(1):67-73.
Comparative studies in expression of model bacterial β-galactosidase gene in culture of murine fibroblasts and during direct injection into the animal liver
1Varzanova I. S., 1Neborachko L. N., 1Shpilevaya S. P., 1Kosletsky I. E., 1Stolyar T. V., 1Sukhorada E. M., 1Lukash L. L., 1Kordium V. A.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


A vehicle with a model gene of the bacterial b-galactosidase has been constructed on the basis of DNA of hepatitis B virus. Comparative studies in the expression of E. coli lacZ gene under the control of promoters of hepatitis B virus and early promoter of SV40 virus in culture of murine fibroblasts (Ltk- cells) and in murine liver cells with immediate introduction of genetic material into liver by injection as part of liposomes have been conducted. The bacterial β-galactosidase gene under the control of promoter of surface antigen hepatitis B virus gene has been expressed much more efficiently in these cells


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