Biopolym. Cell. 1990; 6(1):91-95.
Genome and Its Regulation
Accumulation of strand breaks in DNA fraction containing transcribed sequences in quiescent and starving cells
1Sjakste N. I., 1Budylin A. V.
  1. Research Institute of Experimental and Clinical Medicine, Ministry of Public Health, Latvian SSR
    Riga, USSR


Cellular DNA was separated in fractions containing transcribed sequences (DNAI), untranscribed sequences (DNAII) and "potentially active" sequences (DNAIII) by means of phenol-salt extraction and was subjected to electrophoresis under neutral conditions. Total DNA of quiescent P388 and 4/21 cells contained the great number of short fragments absent in DNA of proliferating cells. After fractionation the short fragments were revealed in DNAI. DNA strand breaks concentrated in DNAI fraction accumulate also, in the starving (incubated in ascitic liquid) Ehrlich ascite cells.


[1] Carpenter G. Properties of the receptor for epidermal growth factor. Cell. 1984;37(2):357-8.
[2] Adler VV, Kracil'nikov MA, Bochkarev GIu, Shapot VS. Early changes in template DNA structure following glucocorticoid injection. Biokhimiia. 1982;47(6):915-20.
[3] S'iakste NI. The role of DNA breaks in the regulation of cell proliferation, differentiation and aging. Ontogenez. 1987;18(3):229-38.
[4] Sjakste NI, Sjakste TG. Detachment of DNA from the nuclear matrix in growth-arrested transformed chinese hamster fibroblasts. Biopolym. Cell. 1986; 2(1):50-2.
[5] Wielckens K, George E, Pless T, Hilz H. Stimulation of poly(ADP-ribosyl)ation during Ehrlich ascites tumor cell "starvation" and suppression of concomitant DNA fragmentation by benzamide. J Biol Chem. 1983;258(7):4098-104.
[6] Strazhevskaia NB, Struchkov VA. Various structural-functional states of DNA in animal cells before and after gamma-irradiation. Radiobiologiia. 1971;11(5):649-55.
[7] Dashkevich VS, Arshynova TV. Isolation of replicating DNA fraction of regenerating rat liver with non-detergent phenol method. Izv Sib Otd Akad Nauk SSSR. 1973; 15(3):133-5.
[8] Dashevich VS, Kuzmenko AP, Skobeleva NA, Frolova L Iu, Salganik RI. Transition of globin genes from the transcriptionally active DNA fraction into the fraction of potentially active DNA during the differentiation of reticulocytes into erythrocytes in birds. Dokl Akad Nauk SSSR. 1983; 270(5):1236-38.
[9] Freeman SE, Blackett AD, Monteleone DC, Setlow RB, Sutherland BM, Sutherland JC. Quantitation of radiation-, chemical-, or enzyme-induced single strand breaks in nonradioactive DNA by alkaline gel electrophoresis: application to pyrimidine dimers. Anal Biochem. 1986;158(1):119-29.
[10] Warters RL, Lyons BW, Chiu SM, Oleinick NL. Induction of DNA strand breaks in transcriptionally active DNA sequences of mouse cells by low doses of ionizing radiation. Mutat Res. 1987;180(1):21-9.
[11] Han S, Udvardy A, Schedl P. Transcriptionally active chromatin is sensitive to Neurospora crassa and S1 nucleases. J Mol Biol. 1984;179(3):469-96.
[12] Votrin II, Khodarev NN, Basnak'ian AG. Endonucleases as a tool for the analysis of the structural and functional organization of chromatin. Vestn Akad Med Nauk SSSR. 1983;(3):88-95.
[13] Chikhirzhina GI, Bubiakina VV, Shvartsman AL, Gaitskhoki VS. Distribution of transcription-active DNA sequences in rat liver chromatin during fragmentation with endogenous DNAses. Mol Biol (Mosk). 1987;21(1):125-31.
[14] Gilmour DS, Pflugfelder G, Wang JC, Lis JT. Topoisomerase I interacts with transcribed regions in Drosophila cells. Cell. 1986;44(3):401-7.
[15] Zhang LH, Mui SC, Todt JC, Strauss PR. Role for topoisomerases in the release of DNA into the detergent-soluble fraction of eukaryotic cells. Proc Natl Acad Sci U S A. 1986;83(16):5871-4.
[16] Sirauss P. R. Detergent soluble DNA is enriched in sequences being actively transcribed. J Cell Biol. 1987; 105(4, pt. 2):69a.