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Gene-Engineering Biotechnology
Excision of HeLa qenomic repeated sequences as a possible way to produce minicircular DNA
1Telegeev G. D., 1Zozulia I. V., 1Maliuta S. S.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Using cloned minicircular DNA(mcDNA) of HeLa cells a number of its genomic copies has been studied. It is found that both probes have chromosome homology and are presented at least by 800-900 copies. The cycloheximide treatment and «ageing» in vitro results in a 0.2-2-fold decrease of its genomic copies with simultaneous increase in Cytoplasmic fraction. It is explained by means of recombination and excision of repeats in the circular form.


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