Biopolym. Cell. 1990; 6(1):103-106.
Short Communications
Rauwolfia genome rearrenqements during culturing in vitro
1Solovjan V. T., 1Zakhlenjuk O. V., 1Kunakh V. A.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Intact Rauwolfia plant and cultivated cell genomes have been comparatively studied. The dot hybridization analysis has shown that there are significant genome rearrangements for R. serpentina callus cultivated for a long time rather than for R. serpentina and R. verticillata calluses cultured for a short time. Restriction analysis has revealed the genome rearrangements both in the primary calluses of R. serpentina and R. verticillata and in the long subcultured R. serpentina cells. It is concluded that genome rearrangements can occur at the early stages of cultivation, but the process of subsequent subculturing in vitro leads to more significant changes in genome.


[1] Solov'yan VT, Kunakh VA, Vershinin AV, Shumnyy VK. Comparison of the degree of DNA homology and quantity of repeated sequences in intact plants and Rauwolfia serpentina Benth cultured cells. Dokl akad nauk sssr. 1986; 287(4):998-1000.
[2] Solovyan VT, Kostenyuk IA, Kunakh VA. Genome changes in Rauwolfia serpentina Benth. cultivated in vitro. Genetika. 1987; 23(7):1200-8.
[3] Nucleic acid hybridization. A practical approach. Eds B. D. Hames, S. J. Higgins. New York : LRL press, 1986. 225 p.
[4] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab. press, 1982 545 p.