Biopolym. Cell. 1992; 8(6):50-54.
Viruses and Cell
Comparison of solid-phase A-protein immune electron microscopy, direct electron microscopy and dot-ELISA for detection of transmissible gastroenteritis virus in feces
1, 2Boulanger A. A., 1, 3Ivanskaya N. V.
  1. Ukrainian Scientific Research Veterinary Institute
    30, Donetska Str., Kyiv, Ukraine, 03151
  2. Consejo de Desarrollo Cientifico y Humanistico de la Universidad
    Universidad Central de Venezuela
    Av. Principal de La Floresta, Qta. Silenia, Caracas, Venezuela
  3. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The authors have shown the solid-phase A-protein immune electron microscopy permits, to detect the fecal coronavirus with the highest sensitivity (54.9 % of 171 fecal samples studied) comparing to direct electron microscopic examination (39.1 %). It is possible to identifity transmissible gastroenteritis virus and to differ it from other virus particles. Dot-ELlSA method has shown to demonstrate viral antigen in 51.4 % of the 171 samples investigated. So the solid-phase immune electron microscopy and dot-ELLSA are convenient for the veterinary diagnostics.


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