Biopolym. Cell. 1992; 8(6):55-57.
Gene-Engineering Biotechnology
Cloning in the laclq of pTTQ plasmids: «reverse» colour reaction
1Alexeyev M. F.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The method for cloning Mlul-, BclI-, ApaI-, BssHI- and EcoRV-DNA fragments in the laclq gene of pTTQ plasmids is described. Recombinant clones are selected as coloured in blue colour colonies on plates containing ampicillin and X-gal.


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