Biopolym. Cell. 2005; 21(4):365-369.
Получение бифункционального полимерного носителя на основе силикагеля для синтеза З'-меченных олигонуклеотидов
1Дубей Л. В., 1Дубей И. Я.
  1. Институт молекулярной биологии и генетики НАН Украины
    ул. Академика Заболотного, 150, Киев, Украина, 03680


Описан способ получения силикагельного полимерного носите­ ля с линкером, содержащим защищенные амино- и гидроксильную группы В качестве эффективного активирующего реаген­та на нескольких стадиях синтеза полимера использовали гексафторфосфат І-бензотриазолилокси-N',N',N'',N'-тетраметилурония в присутствии 1-гидроксибензотриазола. Такой бифункциональный носитель удобен для получения З'-амино-модифицированных олигонуклеотидов для пост-синтетиче­ской конъюгации и для прямого твердофазного синтеза З'-ме­ченных олигонуклеотидов. Смнтезирован флуоресцеин-модифицированный силикагельный полимер
Keywords: полимерные носители, ониевые реагенты, меченые олигонуклеотиды


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