Biopolym. Cell. 1986; 2(5):246-251.
http://dx.doi.org/10.7124/bc.0001BD
Структура та функції біополімерів
Білковий блотинг
1Маргуліс Б. А.
  1. Інститут цитології АН СРСР
    Ленінград, СРСР

Abstract

Метод білкового блотингу активно використовується в молекулярно-біологічних дослідженнях для аналізу взаємодій типу білок–ДНК і білок–білок, включаючи визначення специфічності антигенів і антитіл. Розглянуто різні етапи блотингу – перенесення білків на мембрани, обробка блотів, створення імунних комплексів на репліках і фарбування блотів. Представлено два приклади застосування імуноблотингу для аналізу специфічності антитіл до інтерферонів людини.
Повний текст: (PDF, російською)

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