Biopolym. Cell. 2007; 23(1):21-27.
Молекулярна та клітинна біотехнології
Ендофiтнi бактерiї картоплi in vitro, активованi екзогенними непатогенними бактерiями
1Подоліч О. В., 2Арданов П. Є., 2Вознюк Т. М., 2Ковальчук М. В., 2Данильченко О. В., 3Лащевський В. В., 4Лященко С. А., 2Козировська Н. О.
  1. Інститут агроекології і природокористування НААН України
    вул. Метрологічна 12, Київ, Україна, 03680
  2. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680
  3. Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України
    вул. Академіка Заболотного, 154, Київ, Україна, 03680
  4. Інститут картоплярства УААН
    вул. Чкалова, 22, смт. Немiшаєве, Бородянський р-н, Київська обл. Україна, 07853

Abstract

Мiкробiологiчним i незалежним вiд культивування бактерiй методами показано присутнiсть як культивованих, так i некультивованих форм ендофiтних бактерiй у рослинах - регенерантах картоплi, якi вирощують за умов in vitro. Введення ризобактерiй Pseudomonas fluorescens IМБГ163 у рослини картоплi паралельно з рослинним матерiалом у культуру in vitro збiльшувало кiлькiсть видiв ендофiтних бактерiй корiння першого вегетативного поколiння рослин, але при подальшому клональному мiкророзмноженнi рослин кiлькiсть виявлених ендофiтiв зменшувалася. Ендофiтнi iзоляти сортiв картоплi Загадка та Нiгру, активованi ризобактерiєю, виявляли кориснi для рослин властивостi.
Keywords: ендофiтнi бактерiї, картопля in vitro, бiотичний стресор, Pseudomonas fluorescens IМБГ163

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