Biopolym. Cell. 2007; 23(2):137-142.
Біоорганічна хімія
Синтез 3'- та 3,5-модифікованих олігонуклеотидів на функціоналізованому силікагелі «Силохром-2»
1Дубей І. Я., 1Дубей Л. В., 2Федоряк Д. М.
  1. Інститут молекулярної біології і генетики НАН України
    Вул. Академіка Заболотного, 150, Київ, Україна, 03680
  2. Інститут біоорганічної хімії та нафтохімії НАН України
    вул. Мурманська, 1, Київ, Україна, 02094

Abstract

Описано отримання З'-моно- та 3' ,5' -біс-мічених олігонуклеотидів на новому полімерному носієві з використанням мікросферичного силікагелю «Силохром-2», функціоналізованому флуоресцеїном. На цьому полімері твердофазним фосфітамідним методом синтезовано олігонуклеотид (dT)15, шр містить залишок барвника на 3'-кінці Постсинтетичною 5'-модифікацією З'-конюгата вдалося отримати олігомер, мічений двома молекулами флуоресцеїну. У майбутньому це буде використано для синтезу флуоресцентних зондів для детекції нуклеїнових кислот.
Keywords: олігонуклеотидні кон'югати, флуоресцентні мітки, твердофазний синтез, функціоналізація

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