Biopolym. Cell. 2015; 31(3):161-173.
Огляди
Регуляція ліпоксигеназ in vivo та in vitro сполуками ліпідної природи
1Скатерна Т. Д., 1Копіч В. М., 1Харитоненко Г. І., 1Харченко О. В.
  1. Інститут біоорганічної хімії та нафтохімії НАН України
    вул. Мурманська, 1, Київ, Україна, 02660

Abstract

Ліпоксигенази (ЛО) відомі як одні з ферментів перекисного окислення ліпідів. Більшість ЛО є розчинними ферментами та характеризуються аффіністю до мембран. Транслокація ферменту з цитозолю на мембранну поверхню одна з стадій регуляції рівня продуктів ліпоксигеназного каталізу у клітині. Сорбція на мембранну поверхню описана для більшості ЛОз з рослинних та тваринних джерел. Даний огляд представляє дані по регуляції ЛОз активності природніми та хімічно модифікованими речовинами ліпідної природи. Здат­ність ліпідів регулювати ЛО активність може здійснюватись через: білок-ліпідні взаємодії C2 домену з мембраною, зміну аффінності ферменту, транслокацію ЛОз, алостеричну регуляцію, збільшення селективності до субстрату. Регуляторний вплив активної сполуки на ферментативну активність залежить від рівня ліпофільності ефекторів. При поясненні ліпоксигеназного каталізу необхідно враховувати вплив мікрооточення ферменту на рівень ЛО.
Keywords: ліпоксигеназа, алостерична регуляція, фосфоліпіди, інгібування, активація

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