Biopolym. Cell. 1995; 11(3-4):82-87.
Obtaining and molecular biological analysis transgenic pea plants, contained the maize Ds-element
1Rachek L. I., 1Storozhenko S. V., 1Kuchuk N. V.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680


Pea plants have been transformed with the gene construction containing Ds-element of Ac/Ds maize transposon system. The construction was introduced into pea plants using Agrobaclerium tumefaciens-mediaied transformation. Several transgenic plants have been selected on the nutritional medium contained the kanamycinsulphate as a selective agent. Concentration of NPTII protein was determined by ELISA assay. Using PCR-analyses and Southern blotting hybridization an integration of Ds-element in plant genome has been confirmed.


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